Mark Macbeth, Ph.D
Associate Professor of Biochemistry
School
School of Arts and Sciences
Department
School of Arts and Sciences
Office Phone Office Phone: 617.274.3337
About
Dr. Mark Macbeth earned his PhD in Biochemistry and Molecular Biology from the University of Chicago and completed postdoctoral research at the Howard Hughes Medical Institute at the University of Utah. Prior to joining MCPHS, he served as an Associate Professor in the Department of Chemistry and Biochemistry at Butler University.
At MCPHS, Dr. Macbeth teaches biochemistry and biotechnology laboratory courses and has developed advanced coursework focused on RNA vaccines, RNA-mediated gene silencing, cellular signaling, and protein structure prediction. His research uses X-ray crystallography, enzyme kinetics, and binding studies to investigate RNA-protein interactions and enzyme catalysis.
Education
- PhD, Biochemistry and Molecular Biology – University of Chicago
- BS, Biological Sciences – University of Vermont
- Postdoctoral Associate, Howard Hughes Medical Institute and University of Utah School of Medicine
Research Interests
- RNA editing
- RNA and Protein Structure
- X-ray Crystallography
- Molecular recognition
- Enzyme catalysis
- Enzymes for industrial biocatalysts, environmental remediation, and drug development
Programs
- Biotechnology (BS)
- Chemistry (BS)
- Health Sciences (BS)
- Health Sciences (BS)—Degree Completion
- Health Sciences (DHSc)
- Health Sciences (MHS)
- Health Sciences Pathway: Acupuncture (BS/MAC)
- Health Sciences Pathway: Occupational Therapy (BS/MSOT)
- Medical and Molecular Biology (BS)
- Premedical Health Studies (BS)
- Premedical Health Studies Pathway: Medicine (BS/MD with SGU)
- Premedical Health Studies Pathway: Dental Medicine (BS/DMD)
- Premedical Health Studies Pathway: Optometry (BS/OD)
- Premedical Health Studies Pathway: Osteopathic Medicine (BS/DO with LECOM)
- Premedical Health Studies Pathway: Osteopathic Medicine (BS/DO with ATSU)
- Premedical Health Studies Pathway: Physician Assistant Studies (BS/MPAS)
- Premedical Health Studies Pathway: Veterinary Medicine (BS/DVM with SGU)
- Public Health (BS)
- Public Health (MPH)
- Public Health Graduate Certificate
Featured Affiliations
The RNA Society
WebsiteAmerican Society for Biochemistry and Molecular Biology
WebsiteReview Editor, Frontiers in Molecular Biosciences
WebsiteEducation
- PhD, Biochemistry and Molecular Biology – University of Chicago
- BS, Biological Sciences – University of Vermont
- Postdoctoral Associate, Howard Hughes Medical Institute and University of Utah School of Medicine
Research Interests
- RNA editing
- RNA and Protein Structure
- X-ray Crystallography
- Molecular recognition
- Enzyme catalysis
- Enzymes for industrial biocatalysts, environmental remediation, and drug development
Programs
- Biotechnology (BS)
- Chemistry (BS)
- Health Sciences (BS)
- Health Sciences (BS)—Degree Completion
- Health Sciences (DHSc)
- Health Sciences (MHS)
- Health Sciences Pathway: Acupuncture (BS/MAC)
- Health Sciences Pathway: Occupational Therapy (BS/MSOT)
- Medical and Molecular Biology (BS)
- Premedical Health Studies (BS)
- Premedical Health Studies Pathway: Medicine (BS/MD with SGU)
- Premedical Health Studies Pathway: Dental Medicine (BS/DMD)
- Premedical Health Studies Pathway: Optometry (BS/OD)
- Premedical Health Studies Pathway: Osteopathic Medicine (BS/DO with LECOM)
- Premedical Health Studies Pathway: Osteopathic Medicine (BS/DO with ATSU)
- Premedical Health Studies Pathway: Physician Assistant Studies (BS/MPAS)
- Premedical Health Studies Pathway: Veterinary Medicine (BS/DVM with SGU)
- Public Health (BS)
- Public Health (MPH)
- Public Health Graduate Certificate
Publications
Jackson, K., Hoff, R., Wright, H., Wilkinson, A., Brewer, F., Williams, A., Whiteside, B., Macbeth, M.R., Wilson, A.W. (2024) An Analysis of Protein Crystals Grown under Microgravity Conditions. Crystals. 14:652-663.
Klimkowski, V.J. and Macbeth, M.R., (2023) Proteopedia Entry: Histone Modifying Enzymes. Journal of Biochemistry and Molecular Biology Education. 51(5):586-587.
Bowles, I.E., Pool, E.H., Lancaster, B.S., Lawson, E.K., Savas, C.P., Kartje, Z.J., Severinac, L., Cho, D.H., Macbeth, M.R., Johnson, R.J., and Hoops, G.C. (2021) Transition metal cation inhibition of Mycobacterium tuberculosis esterase RV0045C. Protein Science 30(8):1554-1565.
Ransey, E., Paredes, E., Dey, S. K., Das, S. R., Heroux, A., and Macbeth, M. R. (2017) Crystal structure of the Entamoeba histolytica RNA lariat debranching enzyme EhDbr1 reveals a catalytic Zn(2+) /Mn(2+) heterobinucleation. FEBS Letters 591, 2003–2010.
Truschel, S. T., Zhang, M., Bachert, C., Macbeth, M. R., and Linstedt, A. Allosteric regulation of GRASP protein-dependent Golgi membrane tethering by mitotic phosphorylation. (2012) J. Biol. Chem. 287: 19870-19875.
Goodman, R. A., Macbeth, M.R. and Beal, P.A., ADAR Proteins: Structure and Catalytic Mechanism. (2012) Curr. Top. Microbiol. Immunol. 353: 1-33.
Truschel, S. T., Sengupta, D., Foote, A., Heroux, A., Macbeth, M. R., and Linstedt, A. D., Structure of the membrane tethering GRASP domain reveals a unique PDZ Ligand interaction that mediates Golgi biogenesis. (2011) J. Biol. Chem. 286: 20125-20129.
Macbeth, M. R., and Bass B. L., Large-Scale Overexpression and Purification of ADARs from Saccharomyces Cerevisiae for Biophysical and Biochemical Studies (2007) Meth. Enzymol. 424: 287-297.
Haudenschild, B. L., Maydanovych, O., Véliz, E. A., Macbeth, M. R., Bass, B. L., and Beal, P. A., A transition state analog for an RNA-editing reaction (2004) J. Am. Chem. Soc. 126: 11213-11219.
Macbeth, M. R., Schubert, H. L., VanDemark, A. P., Lingam, A. T., Hill, C. P., and Bass, B. L., Inositol Hexakisphosphate Is Bound in the ADAR2 Core and Required for RNA Editing (2005) Science 309: 1534-1539.
Macbeth, M. R., Lingam, A. T., and Bass, B. L., Evidence for auto-inhibition by the N-terminus of hADAR2 and activation by dsRNA binding, RNA (2004) 10:1563-1571.
Macbeth, M. R., and Wool, I. G., The phenotype of mutations of G2655 in the Sarcin/Ricin domain of 23S Ribosomal RNA, J. Mol. Biol. (1999) 285:965-975.
Macbeth, M. R. and Wool, I. G., Characterization of in vitro and in vivo mutations in non-conserved nucleotides in the Ribosomal RNA recognition domain for the ribotoxins Ricin and Sarcin and the translation elongation factors, J. Mol. Biol. (1999) 285:567-580.